The cloning and expression of the two types of kisspeptin gene in hard-lipped barb (Osteochilus vittatus)

Abstract. Prayogo NA, Rahayu SC, Sugito PPA, Meyanadewi SB, Sukardi P, Rositas RE, Bessho Y. 2023. The cloning and expression of the two types kisspeptin gene in hard-lipped barb (Osteochilus vittatus). Biodiversitas 24: 2797-2804. Hard-lipped (Osteochillus vittatus Valenciennes, 1842) is a native Indonesian species with many benefits for economic, aquaculture, environmental sustainability. Several studies showed that had high reproductive potential, playing role hormone production GnRH secretion. Kisspetin also important gonad maturation fish. Therefore, this study aimed to determine structure Kiss-1 Kiss-2 genes, as well hypothalamus gonads each phase female development.The procedure involved several stages, including organ preparation, isolation, sequence identification, measurement cGnRH expression. was obtained, nucleotide product length 213, coding 89 amino acids. Meanwhile, consisted 230 nucleotides, 76 acids, sequences observed 70-80% other fish species. results values genes were higher compared gonads, lowest adult highest Juvenile phase. This information will lead us use increase reproduction.